壮医祛毒通养方对多囊卵巢综合征大鼠胰岛素抵抗的作用机制OACHSSCD

Objective:To investigate the mechanisms of the Zhuang medicine Qudu Tongyang Formula in rats with polycystic ovary syndrome and insulin resistance(PCOS-IR).Methods:Sixty female Sprague Dawley(SD)rats were randomly divided into a blank control group,a model control group,a positive control group,and low-,medium-,and high-dose Qudu Tongyang Formula groups,with 10 rats in each group.Except for the blank control group,all other groups were subjected to a high-fat diet combined with letro-zole gavage to establish the PCOS-IR model.After 21 days of intervention,estrous cycle changes were observed.Fasting blood glu-cose(FBG),fasting insulin(FINS),luteinizing hormone(LH),follicle-stimulating hormone(FSH),testosterone(T),and estradiol(E2)levels were measured,and the insulin resistance index(HOMA-IR)was calculated.The expression levels of phosphatidyli-nositol 3-kinase(PI3K)/protein kinase B(AKT)/glucose transporter 4(GLUT4)signaling pathway-related molecules in ovarian tissue were detected.Ovarian organ coefficients and pathological morphology were evaluated.Results:Compared with the blank con-trol group,the model control group showed estrous cycle disorder,polycystic ovarian changes,and reduced granulosa cell layers.FBG,FINS,HOMA-IR,LH,T levels,and ovarian coefficients were significantly increased(P＜0.05),while E2 and FSH levels and the mRNA expression of PI3K,AKT,GLUT4,as well as the protein expression of PI3K,AKT,p-AKT,and GLUT4,were significant-ly decreased(P＜0.05).Compared with the model control group,all dose groups of the Qudu Tongyang Formula showed improve-ment in ovarian pathology,with reduced cystic follicles and thickening of the granulosa cell layer.FBG,FINS,HOMA-IR,LH,T lev-els,and ovarian coefficients were significantly decreased(P＜0.05),while E2,FSH levels,and the above pathway-related mRNA and protein expression levels were significantly increased(P＜0.05).Conclusion:The Zhuang medicine Qudu Tongyang Formula can improve insulin resistance and alleviate polycystic ovarian changes in PCOS rats.Its mechanism may be related to activation of the PI3K/AKT/GLUT4 signaling pathway.