二十味肉豆蔻丸对阿尔茨海默病小鼠的治疗作用及机制OACHSSCD

Objective:To investigate the effects of Ershiwei Roudoukou Pills on learning and memory abilities and depressive-like behaviors in 5 familial Alzheimer's disease(5xFAD)transgenic mice.Methods:The 5xFAD transgenic mice were randomly divided into a model group,a positive drug group,and low-,medium-,and high-dose Ershiwei Roudoukou Pills groups,with wild-type(WT)mice serving as the control group.Mice in the control and model groups were administered ultrapure water.The positive drug group was administered a suspension of donepezil and memantine.The low-,medium-,and high-dose Ershiwei Roudoukou Pills groups were administered Ershiwei Roudoukou Pills suspensions for an intervention period of 334 days.Behavioral experiments were used to assess learning and memory abilities and depressive-like behaviors.Immunohistochemistry was used to detect amyloid β-protein 1-42(Aβ1-42)deposition in the brain.Enzyme-linked immunosorbent assay(ELISA)was used to measure corticotropin-releasing hormone(CRH),gonadotropin-releasing hormone(GnRH),thyrotropin-releasing hormone(TRH),thyroid-stimulating hormone(TSH),and corticosterone(CORT).Radioimmunoassay was used to determine adrenocorticotropic hormone(ACTH),follicle-stim-ulating hormone(FSH),luteinizing hormone(LH),estradiol(E2),testosterone(T),triiodothyronine(T3),and thyroxine(T4).Pearson correlation analysis was used to evaluate correlations among indicators.Results:Compared with the model group,mice in the Ershiwei Roudoukou Pills groups showed improved learning and memory abilities,alleviated depressive-like behaviors,and re-duced average optical density of Aβ1-42inthe brain(P＜0.05),as well as decreased levels of ACTH,FSH,LH,and TSH(P＜0.05).Correlation analysis showed that hormone levels in brain regions of 5xFAD mice were significantly correlated with learning and memory abilities,depressive-like behaviors,and Aβ1-42 deposition in these regions(|r|＞0.5).Conclusion:Ershiwei Roudouk-ou Pills can improve learning and memory abilities and alleviate depressive-like behaviors in 5xFAD mice,and the mechanism may be related to regulation of neuroendocrine function.