扶阳复圆汤对抑郁模型大鼠肠道菌群及代谢作用的影响OACHSSCD

Objective:To investigate the antidepressant mechanisms of Fuyang Fuyuan Decoction based on 16S ribosomal DNA(16S rDNA)sequencing and non-targeted metabolomics.Methods:Forty male Sprague Dawley(SD)rats were randomly divided into a blank group,a model group,a fluoxetine group,and a Fuyang Fuyuan Decoction group.A depression model was established u-sing solitary housing combined with chronic unpredictable mild stress(CUMS).After 2 weeks of intervention,depressive-like be-haviors were evaluated using behavioral tests,including the sucrose preference test,open field test,and tail suspension test.Nissl staining and hematoxylin-eosin(HE)staining were used to observe pathological changes in hippocampal neurons and colonic tis-sue,respectively.Gut microbiota and fecal metabolites were analyzed using 16S rDNA sequencing and LC-MS-based non-targeted metabolomics.Spearman correlation analysis was performed to explore the relationships between gut microbiota and metabolites.Re-sults:Compared with the blank group,the model group showed significantly decreased sucrose preference,body weight gain,and lo-comotor activity distance,along with prolonged immobility time(P＜0.01).Fuyang Fuyuan Decoction intervention significantly im-proved these behavioral indicators(P＜0.05 or P＜0.01).The 16S rDNA sequencing showed that Fuyang Fuyuan Decoction regu-lated the Firmicutes/Bacteroidetes(F/B)ratio and modulated the abundance of Actinobacteria and Proteobacteria.Non-targeted metabolomics identified 137 differential metabolites,which were mainly enriched in choline metabolism and glycerophospholipid metabolism pathways.Microbiota-metabolite correlation analysis revealed that genera such as Campylobacter,Defluviitaleaceae UCG-011,and other uncultured taxa were significantly associated with depression-related metabolites.Conclusion:Fuyang Fuyuan Decoction can improve depressive-like behaviors by regulating gut microbiota composition,modulating the F/B ratio,and influen-cing choline and glycerophospholipid metabolic pathways.Its antidepressant mechanism is closely associated with microbiota-metab-olite interactions.