土茯苓对溃疡性结肠炎小鼠的治疗作用研究OACHSSCD

Objective:To investigate the therapeutic effects and molecular mechanisms of Smilax glabra Roxb.(SGR)in a mouse model of ulcerative colitis(UC),focusing on gut microbiota remodeling and mucosal immune homeostasis.Methods:UC was in-duced in mice by dextran sulfate sodium(DSS),followed by prophylactic oral administration of SGR at a human-equivalent dose.Disease severity was evaluated by body weight,disease activity index(DAI),colon length,and histopathology.Goblet cell function and mucin secretion were assessed using Alcian blue/periodic acid-Schiff(AB/PAS)staining.Immune cell populations and inflam-matory mediator expression were measured by quantitative real-time PCR,multiplex bead-based assays,and immunohistochemistry.Cecal contents were analyzed by 16S rRNA gene sequencing to assess gut microbiota composition.Results:SGR markedly alleviated colonic inflammation,preserved goblet cell function,and restored mucus barrier integrity.It effectively suppressed excessive macro-phage activation,restored γδT cell-epithelial cell interactions disrupted by colitis,and reduced overall inflammatory responses.Gut microbiota analysis revealed that SGR increased microbial diversity,enhanced the abundance of beneficial taxa such as Bacte-roides,suppressed pathogenic anaerobes,and modulated specific functional bacterial communities,thereby correcting dysbiosis in UC mice.Conclusion:SGR exerts protective effects against UC by reshaping gut microbiota and restoring mucosal immune homeo-stasis,indicating its potential as a therapeutic candidate for inflammatory bowel disease.