红花黄色素对帕金森病大鼠的改善效果及机制研究OACHSSCD

Objective:To investigate the intervention effects of safflower yellow pigment on Parkinson's disease(PD)rats via the protein kinase B(AKT)/glycogen synthase kinase 3β(GSK3β)signaling pathway and to analyze its underlying mechanisms.Methods:A PD rat model was established by injecting 6-hydroxydopamine into the striatum.Sixty-four PD model rats were random-ly assigned using a random number table to a model group,low-and high-dose safflower yellow pigment groups,and a Madopar group,with a separate sham operation group as control.After 4 weeks of continuous intervention,motor ability and motor disorders were assessed by open field and rotation tests.Pathological changes in the substantia nigra were observed using hematoxylin-eosin staining.Serum inflammatory markers[interleukin-6(IL-6),IL-18]and substantia nigra oxidative stress markers[superoxide dis-mutase(SOD),malondialdehyde(MDA)]were measured by enzyme-linked immunosorbent assay.The mRNA and protein levels of AKT and GSK3β in the substantia nigra were detected using quantitative polymerase chain reaction and Western blot.Results:Compared with the sham operation group,the model group showed severe pathological damage in the substantia nigra,significantly decreased number of squares crossed,SOD levels,and AKT and GSK3β mRNA and protein levels,and significantly increased rota-tion counts,serum IL-6,IL-18,and substantia nigra MDA levels(P＜0.05).Compared with the model group,safflower yellow pig-ment treatment dose-dependently reduced pathological damage,increased the number of squares crossed,SOD levels,and AKT and GSK3β mRNA and protein levels,and decreased rotation counts,serum IL-6,IL-18,and MDA levels in a dose-dependent manner(P＜0.05).Conclusion:Safflower yellow pigment improves motor ability,motor disorders,and substantia nigra pathology in PD rats,reduces serum inflammation and oxidative stress,and its mechanism may involve activation of the AKT/GSK3 β signaling path-way.