甘草泻心汤加减对小鼠口腔溃疡治疗作用及其主要成分相关性研究OACHSSCD
Therapeutic Effects of Modified Gancao Xiexin Decoction on Oral Ulcers in Mice and Correlation Analysis of its Main Components
目的:研究甘草泻心汤加减对小鼠口腔溃疡的治疗作用,及其化学成分与促进溃疡愈合的相关性.方法:以水提法、水提醇沉法、乙醇回流法、水提絮凝法等4种方法提取甘草泻心汤加减获得药液.建立小鼠口腔溃疡模型,比较不同方法提取物对小鼠口腔溃疡愈合的促进作用.组织切片观察溃疡组织形态变化,酶联免疫吸附试验(ELISA)检测血清中免疫相关因子变化.液相色谱高分辨质谱联用法(LC-MS)分析药液的主要化学成分.根据《中华人民共和国药典》2020年版确定各药材的19种典型物质,检测在不同提取方法所得到的提取物含量.采用偏最小二乘法回归法(PLSR)分析它们与模型小鼠的体质量增长率和溃疡的愈合程度之间的相关度.结果:甘草泻心汤的原方及加减的不同方法提取物,均可以加速小鼠口腔溃疡愈合,降低小鼠体质量减轻速率.其中甘草泻心汤加减的水提絮凝法提取物效果最好,小鼠血清中的粒细胞巨噬细胞集落刺激因子(GM-CSF)含量在溃疡早期从578 pg/mL提高到1 214 pg/mL,在后期溃疡愈合后显著降低到661 pg/mL.LC-MS共鉴定出66种小分子化合物.PLSR分析表明,柚皮素、蒲公英甾醇以及黄芩素与小鼠的体质量增长和溃疡恢复相关性较大.结论:甘草泻心汤加减可以促进口腔溃疡愈合,其中水提絮凝法提取物效果最好,分析表明促进效果是药材中多个成分的共同作用结果.
Objective:To investigate the therapeutic effects of modified Gancao Xiexin Decoction on oral ulcers in mice and to ana-lyze the correlation between its chemical components and ulcer healing.Methods:The modified Gancao Xiexin Decoction was ex-tracted using water extraction,water extraction followed by alcohol precipitation,ethanol reflux,and water extraction followed by flocculation.An oral ulcer model was established in mice to compare the effects of extracts obtained by different methods on ulcer healing.Histological sections were used to observe morphological changes in ulcer tissues,and serum immune-related factors were measured by enzyme-linked immunosorbent assay(ELISA).The main chemical components of the extracts were analyzed using liq-uid chromatography-high-resolution mass spectrometry(LC-HRMS).According to the 2020 edition of the Chinese Pharmacopoeia,19 typical compounds from each herb were identified,and their contents in the extracts were quantified.Partial least squares regres-sion(PLSR)analysis was used to examine correlations between these compounds,body weight gain in mice,and ulcer healing.Re-sults:Extracts from both the original and modified Gancao Xiexin Decoction accelerated oral ulcer healing and reduced weight loss in mice.Among the different extraction methods,the water extraction followed by flocculation showed the best effect.Serum granulo-cyte-macrophage colony-stimulating factor(GM-CSF)levels increased from 578 pg/mL to 1 214 pg/mL during the early stage of ul-cers and decreased to 661 pg/mL after healing.LC-MS identified a total of 66 small molecular compounds.PLSR analysis indicated that naringenin,taraxasterol,and baicalein were most strongly correlated with body weight gain and ulcer recovery.Conclusion:Modified Gancao Xiexin Decoction promotes oral ulcer healing,with the water extraction followed by flocculation method being the most effective.The healing effect is likely due to the combined action of multiple chemical components in the medicinal materials.
肖蕾;杨妞;何陵玲;董路路;王海英;曾晓波
无限极[中国]有限公司,广州,510405中南民族大学生命科学学院,武汉,430074无限极[中国]有限公司,广州,510405无限极[中国]有限公司,广州,510405中南民族大学生命科学学院,武汉,430074武汉大学药学院,武汉,430071
医药卫生
甘草泻心汤加减提取方法口腔溃疡促进修复免疫相关因子活性成分偏最小二乘回归法相关性分析
Modified Gancao Xiexin DecoctionExtraction methodsOral ulcersPromotion of healingImmune-related factorsActive componentsPartial least squares regressionCorrelation analysis
《世界中医药》 2026 (3)
382-391,10
国家自然科学基金面上项目(32571896)无限极(中国)有限公司科学研究基金项目(HPG20220930698).
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