不同制备工艺苓桂术甘汤化学成分分析与方剂配伍合理性分析OACHSSCD

Objective:To compare the similarities and differences in the material basis of Linggui Zhugan Decoction(LGZG)pre-pared using different methods,and to clarify the potential relationship between material distribution and compatibility based on net-work pharmacology.Methods:A targeted determination method for LGZG was established using ultra-high-performance liquid chro-matography coupled with quadrupole time-of-flight tandem mass spectrometry(UHPLC-QTOF-MS/MS).Qualitative and relative quantitative analyses were conducted on traditional benchmark samples and intelligent decoction samples.Key active ingredients were screened,and Gene Ontology(GO)enrichment analysis of targets related to non-alcoholic fatty liver disease(NAFLD)was performed using network pharmacology platforms,including SWISS.Results:Both the traditional benchmark and intelligent decoc-tion samples contained 142 components,with 91.89％overlap,indicating highly similar chemical profiles.Relative quantitative a-nalysis showed that the intelligent decoction process improved the dissolution of Poria cocos components,which were poorly soluble in the traditional water decoction.GO enrichment analysis revealed that P.cocos was associated with the largest number of genes and exhibited the highest betweenness centrality,suggesting it plays the principal therapeutic role,while the other herbs provide synergistic and auxiliary effects.Conclusion:Using UHPLC-QTOF-MS/MS,this study systematically analyzed the similarities and differences in the material basis of LGZG prepared by different methods at both qualitative and relative quantitative levels.Network pharmacology analysis clarified the rationale for LGZG compatibility in treating NAFLD from the perspective of material distribu-tion,providing a reference for chemical composition studies of other traditional Chinese medicine decoctions.