青贮芦苇对肉羊表观消化率、瘤胃发酵参数及菌群结构的影响OA

The trial aimed to investigate the effects of replacing silage corn with silage reed in the diet on apparent digestibility,ruminal fermentation parameters,and microbial community structure in meat sheep.Forty healthy meat sheep(Oula sheep×Bayinbuluke sheep)with an average body weight of(32.00±0.20)kg were randomly divided into two groups:the corn silage group(CON group)and the silage reed group(Treat group).Each group contained 4 replicates,with 5 sheep per replicate.The trial included a 9-day adaptation period followed by a 40-day experimen-tal period.The results showed that:① compared with the CON group,the overall average daily gain(ADG)and average dry matter intake(DMI)of the Treat group were significantly decreased(P<0.05).② The digestibility of dry matter and crude pro-tein in the Treat group was significantly lower than that in the CON group(P<0.05),while the neutral detergent fiber digestibility was significantly higher than in the CON group(P<0.05).③ The Treat group showed significantly higher acetate/total vola-tile fatty acid and acetate/propionate ratios compared to the CON group(P<0.05),whereas the propionate/total volatile fatty acid ratio was significantly lower in the Treat group than in the CON group(P<0.05).④The Chao1 index of the Treat group was significantly increased compared to the CON group(P<0.05).The Treat group exhibited increased abundances of Bacteroidetes and Proteobacteria but a reduced abundance of Firmicutes.LEfSe analysis revealed significant enrichment of Anaerostipes,Bacillus,Pseudomonas,Palu-dibacter,Akkermansia,Chitinophaga,and Corynebacterium in the Treat group.Silage reed replacement of silage corn decreased feed intake and daily gain of meat sheep,increased digestibility of neutral detergent fiber and acetic acid content of meat sheep,transformed rumen fermentation into acetic acid fermentation,increased the abundance of fiber degrading bacteria,optimized rumen function,and silage reed could be used to replace silage corn in a short time in the case of feed resource shortage.