肺腺癌组织中CCT7表达变化的预测与临床验证OA

Objective Based on bioinformatics databases,we predicted the expression changes of chaperonin contai-ning T-complex polypeptide 1 subunit 7(CCT7)in lung adenocarcinoma tissues,and subsequently performed clinical vali-dation.Methods The transcriptome sequencing data of 58 patients with lung adenocarcinoma from the TCGA lung adeno-carcinoma project were downloaded from the GDC database,and the expression changes of CCT7 mRNA in lung adenocarci-noma tissues and adjacent non-cancerous tissues were compared.Clinicopathological staging information of TCGA lung ade-nocarcinoma patients was downloaded from the UCSC Xena database and integrated with the CCT7 mRNA expression profile data to analyze the relationship between CCT7 mRNA expression and clinicopathological staging of lung adenocarcinoma pa-tients.Based on the CCT7 mRNA expression profile data from the TCGA database,lung adenocarcinoma patients were di-vided into the high CCT7 expression group and low CCT7 expression group using the median expression level as the cutoff value,and the relationship between CCT7 mRNA expression and prognosis of lung adenocarcinoma patients was analyzed.Cancer tissue and paired adjacent non-cancerous tissue samples were collected from 30 patients with lung adenocarcinoma who were admitted to The Affiliated Xuzhou Municipal Hospital of Xuzhou Medical University from September 2023 to Au-gust 2025.Real-time quantitative PCR was used to detect CCT7 mRNA in lung adenocarcinoma tissues,and Western blot-ting was used to detect CCT7 protein in lung adenocarcinoma tissues.The relationships between the relative expression lev-els of CCT7 mRNA and protein and the clinicopathological staging of lung adenocarcinoma patients were also analyzed.Results Analysis of the TCGA database showed that the relative expression level of CCT7 mRNA in the lung adenocarcino-ma tissues was higher than that in the adjacent non-cancerous tissues(P<0.05);the relative expression levels of CCT7 mRNA in stage Ⅲ and stage Ⅳ lung adenocarcinoma tissues were both higher than that in stage Ⅰ(both P<0.05).The median overall survival of the high CCT7 expression group was lower than that of the low CCT7 expression group(P<0.05).In the 30 patients with lung adenocarcinoma,the relative expression levels of CCT7 mRNA and protein in the cancer tissues were higher than those in the adjacent non-cancerous tissues(both P<0.05).The relative expression levels of CCT7 mRNA and protein in stage Ⅰ-Ⅱ lung adenocarcinoma tissues were lower than those in stage Ⅲ-Ⅳ(all P<0.05).Conclusion CCT7 is highly expressed in lung adenocarcinoma tissues,and its expression level is associated with the clinicopathological stage and prognosis of patients with lung adenocarcinoma.