肺腺癌组织中TANC2表达变化及其意义OA

Objective To observe the expression changes of TANC2 in lung adenocarcinoma(LUAD)tissues based on bioinformatics databases and to investigate its clinical significance.Methods The expression changes of TANC2 mRNA in LUAD and normal lung tissues were analyzed using the UALCAN database,based on The Cancer Genome Atlas(TC-GA)dataset.The normalized relative protein expression(nRPX)levels of TANC2 protein in LUAD and normal lung tissues were analyzed using the Human Protein Atlas(HPA)database.The relationship between TANC2 mRNA expression and clinicopathological parameters of LUAD patients was analyzed using the UALCAN database.Based on the HPA database,the correlation between the relative expression level of TANC2 mRNA and the prognosis of patients with LUAD was ana-lyzed.Co-expressed genes of TANC2 in LUAD were identified using the LinkedOmics database,followed by KEGG signa-ling pathway enrichment analysis.Results Both TANC2 mRNA expression and TANC2 protein nRPX levels were signifi-cantly higher in the LUAD tissues than in the normal lung tissues(both P<0.001).TANC2 mRNA expression was signifi-cantly higher in female LUAD patients than in male patients,and was also significantly higher in stage III patients than in stage IV patients(all P<0.05).The 5-year survival rate of patients in the high TANC2 mRNA expression group was signifi-cantly lower than that in the low expression group(P<0.05).Co-expression analysis revealed that 5,219 genes were signifi-cantly positively correlated with TANC2 expression(P<0.05),among which DDX42 exhibited the strongest correlation(R=0.611 5).Conversely,5,419 genes were significantly negatively correlated with TANC2(P<0.05),and COMMD7 exhibited the strongest negative correlation(R=-0.545 0).KEGG pathway enrichment analysis revealed that TANC2 might be involved in the activation of multiple signaling pathways,including cancer-related microRNA regulation pathway,adher-ens junction pathway,and thyroid hormone signaling pathway.Conclusions TANC2 is highly expressed in LUAD tissues,and its elevated expression is associated with poor prognosis in LUAD patients.In addition,the expression changes of TANC2 in LUAD are related to DDX42 and COMMD7 genes,and may participate in the regulation of cancer-related signa-ling pathways,such as the microRNA pathway in cancer.