芥菜BjWRKY44基因的克隆及表达分析OA

To clarify the effects of WRKY44 transcript factor in anthocyanidin accumulation in mustard(Brassica juncea),a WRKY transcription factor gene,BjWRKY44,was obtained using near-isogenic lines of purple stalk mustard and green stalk mustard as material.The bioinformatics characteristics of BjWRKY44 pro-tein,the expression patterns of the gene together with their relationships with anthocyanidin content were ana-lyzed.The results showed that the length of genomic DNA and cDNA sequences of BjWRKY44 were 1 516 bp and 1 266 bp,respectively,encoding 421 amino acids.The BjWRKY44 protein contained the conserved do-main of WRKY,localized in nucleus,and was clustered in the same branch with WRKY44 of Brassica napus and Brassica rapa showing close relationships with them.The results of fluorogenic quantitative PCR analysis indicated that the relative expression level of BjWRKY44 in purple mustard was significantly higher than that of green mustard,and its expression level was significantly positively correlated with the anthocyanidin content.The results of protein interaction network analysis induced that BjWRKY44 interacted with proteins involved in anthocyanidin biosynthesis and transportation and MBW complex.In summary,BjWRKY44 might regulate the accumulation of anthocyanidin in B.juncea.This study could provide some theoretical foundation for studying on the function and mechanism of WRKY involved in the accumulation of anthocyanidin in B.juncea.