提高1日龄雏鸡传染性支气管炎活疫苗喷雾免疫定植率的试验研究OA

To optimize the spray immunization procedure for chicks in hatcheries and establish a scientific and standardized method for evaluating immune efficacy.This study used the nucleic acid positive rate of infectious bron-chitis vaccine strain in pharyngeal-palatal swabs of chicks after hatchery spray immunization as the indicator of im-mune colonization rate.By adjusting the spray immunization operation parameters,such as single spray dose and im-munization environmental conditions(temperature,light intensity,etc.),the positive rate of infectious nucleic acid in the pharyngeal and palatal swabs of chicks 3-5 days after different immunization operations was detected,and the influence of each factor on the immunization effect was analyzed.The results showed that spray immunization with a pressure of 0.4 MPa and a spray dose of 14 mL/100chicklings,under conditions of light intensity above 100 lx and room tem-perature of 29-30℃,could achieve better immunization effects.Standard sampling and testing 4-5 days after immu-nization could effectively evaluate the spray immunization effect of infectious bronchitis.