邵阳鸡源奇异变形杆菌分离鉴定及生物学特性研究OA

This study focuses on chicken-derived Proteus mirabilis(PM)in Shaoyang,Hunan,aiming to clarify its epidemiological characteristics,drug-resistant phenotypes,biofilm-forming abilities,and virulence characteristics,thereby providing scientific support for the precise prevention and control of this bacterium in poultry farms and the rational use of antibacterial agents.For the experiment,6 large-scale farms with 75-day-old layer hens in Shaoyang,which had basically consistent ecological conditions,were selected,and 153 anal swab samples were collected.After isolation and culture,biochemical identification,and 16S rDNA sequencing verification to obtain the target strains,systematic tests were conducted on the isolated strains,including determination of biofilm-forming ability,drug sensitivity test,and detec-tion of drug-resistant genes and virulence genes,so as to complete the strain screening and characteristic analysis through-out the process.The results showed that the isolation rate of chicken-derived Proteus mirabilis in Shaoyang was 37.9%(58/153).Most strains exhibited a typical swarming growth phenotype,among which strains with strong biofilm-forming ability accounted for a prominent proportion,reaching 50.0%.The drug sensitivity test indicated that the isolated strains had significant drug-resistant characteristics:the drug resistance rate to meropenem was relatively low(24.14%),while the drug resistance rate to sulfafurazole was as high as 100.00%.The multi-drug resistance phenomenon was serious,with 89.7%(52/58)of the strains being resistant to 8 or more antibiotics.Gene detection revealed that the 58 strains carried a total of 84 types of drug-resistant genes and 56 types of virulence and colonization-related genes.This study indicates that chicken-derived Proteus mirabilis in Shaoyang has shown a composite phenotype of high drug resistance and high virulence.It is urgent to strengthen regional monitoring and explore new precise prevention and control strategies to re-duce its harm to the poultry industry and potential public health risks.