一种中药生发液的毛囊刺激性及防脱生发功效探究OA

This study observed the effects of the same Traditional Chinese Medicine combination on hair papilla cells,keratinocytes,and isolated hair follicles under 7 different extraction methods,and explored which extraction method promotes the effect of promoting hair growth while causing less toxic damage to cells.The MTT assay was used to detect the toxicity and proliferative effects of the seven extraction solutions on dermal papilla cells and keratinocytes.Three extraction solutions with better cell viability enhancement effects at the 72-hour time point in the proliferation experiment were selected for hair follicle culture in vitro,and the growth of hair follicles and changes in hair follicle growth cycle were observed.Finally,the fingerprint spectrum of the Traditional Chinese Medicine formula was constructed using liquid chromatography-mass spectrometry,and the main chemical components were identified.The results show that the extraction method for 7 medicinal herbs with alcohol precipitation,and 3 medicinal herbs with alcohol extraction combined with 15 μL of volatile oil(G extraction solution)has a significant promoting effect on the growth of hair follicles in vitro.It also has a regulatory effect on the growth cycle of hair follicles by prolonging the growth period and slowing the degeneration period,thereby achieving hair growth.