磷限制对莱州湾浮游植物碳氮磷化学计量特征影响的实验研究OA
Study on the Effects of Phosphorus Limitation on the Stoichiometric Characteristics of Carbon,Nitrogen and Phosphorus in Phytoplankton in Laizhou Bay
本论文以氮高磷低、磷限制严重的莱州湾为研究区域,分析了不同氮磷比调控的现场围隔现场实验和典型浮游植物室内培养实验,及不同氮磷比条件下围隔现场实验和室内培养实验过程中浮游植物细胞吸收同化氮磷的化学计量学特征.结果表明,莱州湾浮游植物对氮、磷营养盐的吸收是弹性的.水体处于非磷限制时,浮游植物基本按照Redfield比值(16∶1)吸收营养盐,浮游植物细胞内颗粒有机碳(Particulate organic carbon,POC)/颗粒态氮(Particulate nitrogen,PN)/颗粒态磷(Particulate phosphorus,PP)比接近Redfield比值(106∶16∶1).水体磷限制时,浮游植物对氮奢侈吸收同化,导致浮游植物细胞内POC/PN低于Redfield比值,POC/PP和PN/PP则高于Redfield比值;且随着氮磷比增大,POC/PN比值减小,POC/PP和PN/PP比值则增大.磷限制导致浮游植物细胞内POC/PN/PP比例失衡,导致浮游植物细胞内高碳物质增多,碳同化效率降低.
The study was conducted in the Laizhou Bay,a region characterized by high nitrogen levels,low phosphorus levels,and severe phosphorus limitation.Through in-situ mesocosm experiments regu-lated by nitrogen-to-phosphorus ratios and laboratory cultivation experiments of different phytoplankton types,the stoichiometric characteristics of phytoplankton under varying N/P conditions were analyzed.The results indicate that phytoplankton in Laizhou Bay exhibit flexible absorption of nitrogen and phos-phorus nutrients.Under non-phosphorus-limited conditions,phytoplankton absorb nutrients essentially according to the Redfield ratio(16∶1),and the intracellular ratios of particulate organic carbon(POC)to particulate nitrogen(PN)to particulate phosphorus(PP)closely approximate the Redfield ratio(106∶16∶1).Under phosphorus-limited conditions,phytoplankton luxuriously absorb and assimilate nitrogen,leading to intracellular POC/PN ratios lower than the Redfield ratio,while POC/PP and PN/PP ratios become higher than the Redfield ratio.Furthermore,as the N/P ratio increases,the POC/PN ratio decreases,whereas the POC/PP and PN/PP ratios increase.Phosphorus limitation causes an im-balance in the intracellular POC/PN/PP ratios of phytoplankton,resulting in an increase in high-carbon substances within the cells and a reduction in carbon assimilation efficiency.
夏旦琳;王友华;段晓萌;张明政;李宏观;梁生康;王修林
中国海洋大学化学化工学院,山东 青岛 266100||中国海洋大学海洋化学理论与工程技术教育部重点实验室,山东 青岛 266100中国海洋大学化学化工学院,山东 青岛 266100||中国海洋大学海洋化学理论与工程技术教育部重点实验室,山东 青岛 266100中国海洋大学化学化工学院,山东 青岛 266100||中国海洋大学海洋化学理论与工程技术教育部重点实验室,山东 青岛 266100中国海洋大学化学化工学院,山东 青岛 266100||中国海洋大学海洋化学理论与工程技术教育部重点实验室,山东 青岛 266100中国海洋大学化学化工学院,山东 青岛 266100||中国海洋大学海洋化学理论与工程技术教育部重点实验室,山东 青岛 266100中国海洋大学化学化工学院,山东 青岛 266100||中国海洋大学海洋化学理论与工程技术教育部重点实验室,山东 青岛 266100中国海洋大学化学化工学院,山东 青岛 266100||中国海洋大学海洋化学理论与工程技术教育部重点实验室,山东 青岛 266100
海洋科学
莱州湾磷限制浮游植物化学计量比围隔实验
Laizhou Bayphosphorus limitationphytoplanktonstoichiometric ratiosmesocosm expe-riment
《中国海洋大学学报(自然科学版)》 2026 (6)
66-77,12
国家自然科学基金项目(42576040)崂山实验室科技创新项目(LSKJ202203901)山东省重点研究发展项目(2022QNLM040002)中央高校基本科研业务费专项(20247200)资助Supported by the National Natural Science Foundation of China(42576040)the Laoshan Laboratory Science and Technology Innova-tion Project(LSKJ202203901)the Key Research and Development Project of Shandong Province(2022QNLM040002)the Special Fund for Basic Scientific Research Business Expenses of Central Universities(20247200)
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