OGG1调控心肌细胞增殖的功能研究OA

To investigate the regulatory function of 8-oxoguanine DNA glycosylase 1(OGG1)on cardiomyocyte proliferation,si-OGG1 small interfering RNA(OGG1-si)and OGG1 overexpression adenovirus(OGG1-OE)were transfected to construct primary car-diomyocyte models with OGG1 knockdown and overexpression,respectively.The effi-ciency of knockdown and overexpression was detected by real-time quantitative PCR(qPCR),and the proliferation status of primary cardiomyocytes was evaluated by the ex-pression levels of proliferation markers(Ki67 and pH3).The results show that compared with the control group,the expression of proliferation markers Ki67 and pH3 is signifi-cantly decreased in the OGG1 knockdown group,while both of them significantly increase in the OGG1 overexpression group.This indicates that OGG1 has a crucial regulatory role in cardiomyocyte proliferation,and its overexpression can significantly promote cardio-myocyte proliferation,while knockdown inhibits cardiomyocyte proliferation.