湖羊TNNI3和MOGAT2基因遗传多态性与脂肪沉积性状的关联分析OA
Genetic Polymorphism of the TNNI3 and MOGAT2 Genes and Its Association Analysis with Fat Deposition Traits in Hu Sheep(Ovis aries)
心肌肌钙蛋白I3(troponin I3,TNNI3)通过抑制肌动蛋白-肌球蛋白相互作用调控心肌收缩,是临床诊断心肌梗死的核心标志物;单酰基甘油O-酰基转移酶2(monoacylglycerol O-acyltransferase 2,MOGAT2)主要在小肠中催化单酰甘油与脂肪酸合成甘油二酯,对膳食脂肪的吸收和再酯化过程起关键作用.为探讨湖羊(Ovis aries)TNNI3和MOGAT2基因SNP及其与脂肪沉积性状的关联,为分子辅助育种提供潜在的分子标记.本研究选取1 029只系谱清晰、实验数据记录完整且体格健康的雄性湖羊为实验对象,通过PCR扩增和MassARRAY® SNP基因分型技术对TNNI3和MOGAT2基因的多态性位点进行检测,利用一般线性模型对基因多态性与脂肪沉积相关性状进行关联分析,并检测2个基因在脂肪组织中的表达量.结果显示,在TNNI3和MOGAT2中共检测到2个多态性位点,分别为位于TNNI3基因第1个内含子的TNNI3 g.66954963 T>G和位于MOGAT2基因第2个内含子的MOGAT2 g.58004696 A>G.关联分析结果表明,TNNI3 g.66954963 T>G多态性与尾脂重、肾周脂肪重和肠系膜脂肪重显著相关(P＜0.05),MOGAT2 g.58004696 A>G多态性与肾周脂肪重、肾周脂肪相对重、肠系膜脂肪重和肠系膜脂肪相对重显著相关(P＜0.05),GGTNNI3-GGMOGAT2基因型组合的湖羊群体尾脂重、肾周脂肪重、肾周脂肪相对重、肠系膜脂肪重和肠系膜脂肪相对重均显著低于TTTNNI3-GAMOGAT2基因型群体(P＜0.05);RT-qPCR分析显示,TNNI3基因在肾周脂肪和肠系膜脂肪组织中表达量高,MOGAT2基因在尾脂组织中高表达.综上,TNNI3和MOGAT2基因在湖羊脂肪沉积中有重要作用.本研究为湖羊分子辅助育种提供潜在的分子标记.
Troponin I3(TNNI3),which regulates myocardial contraction by inhibiting actin-myosin interaction and serves as a core biomarker for the clinical diagnosis of myocardial infarction;monoacylglycerol O-acyltransferase 2(MOGAT2)primarily catalyzes the synthesis of diacylglycerol from monoacylglycerol and fatty acids in the small intestine,playing a key role in dietary fat absorption and re-esterification.To investigate the association between SNP in the TNNI3 and MOGAT2 genes and fat deposition traits in Hu sheep(Ovis aries),a total of 1 029 male Hu sheep with clear pedigrees,complete phenotypic records,and healthy conditions were selected as experimental subjects in this study.Polymorphic loci of the TNNI3 and MOGAT2 genes were detected using PCR amplification and MassARRAY® SNP genotyping technology.A general linear model was used for association analysis of fat deposition-related traits,and the tissue expression patterns of the 2 genes were further determined.The results revealed 2 polymorphic sites:TNNI3 g.66954963 T>G,located in the first intron of TNNI3 gene,and MOGAT2 g.58004696 A>G,located in the second intron of MOGAT2 gene.Association analysis indicated that the TNNI3 g.66954963 T>G polymorphism was significantly associated with caudal fat weight,perirenal fat weight,and mesenteric fat weight(P＜0.05).The MOGAT2 g.58004696 A>G polymorphism was significantly associated with perirenal fat weight,relative perirenal fat weight,mesenteric fat weight,and relative mesenteric fat weight(P＜0.05).The combined genotype GGTNNI3-GGMOGAT2 showed significantly lower values for caudal fat weight,perirenal fat weight,relative perirenal fat weight,mesenteric fat weight,and relative mesenteric fat weight compared to the TTTNNI3-GAMOGAT2 genotype group(P＜0.05).RT-qPCR analysis demonstrated that high expression of the TNNI3 gene in perirenal and mesenteric fat,while the MOGAT2 gene was highly expressed in caudal fat.In conclusion,the TNNI3 and MOGAT2 genes played important roles in fat deposition in Hu sheep.This study provides potential molecular markers for molecular-assisted breeding.
周颖慧;马博;张小雪;王维民;田慧彬;徐全忠;马宗武;杨晓斌;张健;曹佩亮;蔡桐
甘肃农业大学 动物科学技术学院,兰州 730070甘肃农业大学 动物科学技术学院,兰州 730070甘肃农业大学 动物科学技术学院,兰州 730070兰州大学 草地农业科技学院,兰州 730000兰州大学 草地农业科技学院,兰州 730000兰州大学 草地农业科技学院,兰州 730000兰州大学 草地农业科技学院,兰州 730000兰州大学 草地农业科技学院,兰州 730000甘肃农业大学 动物科学技术学院,兰州 730070甘肃农业大学 动物科学技术学院,兰州 730070甘肃农业大学 动物科学技术学院,兰州 730070
农业科技
肌钙蛋白I3(TNNI3)单酰基甘油O-酰基转移酶2(MOGAT2)SNP脂肪沉积湖羊
Troponin I3(TNNI3)Monoacylglycerol O-acyltransferase 2(MOGAT2)SNPFat depositionHu sheep
《农业生物技术学报》 2026 (6)
1232-1242,11
国家自然科学基金(32372850)甘肃省研产融合科技攻关赋能计划(25FNNH001)武威市科技重大专项(WW23A03ZDQ001)
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