合作猪CSRP3基因的克隆、生物信息学及组织表达分析OA

The cysteine and glycine rich protein 3(CSRP3)gene,as a key regulator of animal muscle fiber development,plays a core role in muscle growth and functional maintenance.Through using PCR amplification and sequencing,the full-length CDS sequence of CSRP3 gene in Hezuo pig was obtained.Bioinformatics analysis software was used to perform similarity comparisons,phylogenetic tree construction,and domain prediction analysis on the gene sequence.Additionally,qRT-PCR was employed to detect the expression levels of the CSRP3 gene in 11 tissues of Hezuo pigs,including the heart,liver,spleen,and longissimus dorsi muscle,The results showed that the CDS of CSRP3 gene of Hezuo pig was 585 bp and encoded 194 amino acids,there were 4 base mutations,all of them were synonymous mutations.The homologous sequence comparison and phylogenetic tree analysis showed that Hezuo pig had the closest relationship with Duroc and the most distant relationship with Gallus gallus;the molecular weight of 20.94 kD,the isoelectric point(pI)was 8.89,and the coefficient was 39.11,protein with fewer hydrophobic amino acid residues than hydrophilic ones.It had neither a transmembrane structure nor a signal peptide;analysis of its expression characteristics in different tissues showed that this gene had the highest expression level in the heart of Hezuo pigs and the lowest in the liver.This study provides a reference for further functional studies of the CSRP3 gene in Hezuo pigs.