白丁香SoMYB3基因克隆及功能研究OA

Syringa oblata var.alba is a white-flowered variant of Syringa oblata.The MYB transcription factor plays a crucial role in regulating anthocyanin biosynthesis.In this study,the SoMYB3 transcription factor gene was cloned from the petals of S.oblata var.alba.The SoMYB3 gene,belonged to typical R2R3-MYB transcription factor,with an ORF length of 705 bp and encoding 234 amino acids.The phylogenetic analysis showed that SoMYB3 protein was closely related to grape(Vitis vinifera)VvMYBA1 and tea(Camellia sinensis)CsMYB113 protein.SoMYB3 was expressed in roots,stems,leaves,flower buds and flowers of S.oblata var.alba to varying degrees,and the highest expression level was in flowers.The heterologous expression of the SoMYB3 gene promoted the accumulation of anthocyanins in the roots,stems,leaves,flowers,capsules and seeds of tobacco(Nicotiana tabacum),by inducing the expression of anthocyanin 2(NtAN2)(MYB transcription factor)and anthocyanin 1b(NtAN1b)(bHLH transcription factor),and upregulating the gene expression level of chalcone synthase(NtCHS),chalcone isomerase(NtCHI),dihydroflavonol 4-reductase(NtDFR),anthocyanidin synthase(NtANS),UDP-glucose:flavonoid 3-O-glucosyltransferase(NtUFGT),and anthocyanidin reductase(NtANR)in tobacco.In conclusion,the SoMYB3 transcription factor could induce anthocyanin synthesis,and the color of petal in S.oblata var.alba might be regulated by multiple transcription factors.This study provides a scientific basis for the directional improvement of Syringa oblata var.alba flower color.