黄秋葵NCED基因的鉴定及其在果荚中的表达分析OA

Abscisic acid(ABA)serves as a pivotal hormone in plants,regulating the entire process of plant growth,development,and stress responses.Nine-cis-epoxycarotenoid dioxygenase(NCED)acts as the rate-limiting enzyme in ABA biosynthesis,directly determining its biosynthetic efficiency and content.The identification and analysis of the NCED gene in Hibiscus esculentus are crucial for elucidating the mechanism of ABA-mediated regulation of pod senescence.In this study,the HeNCED gene in H.esculentus was identified and analyzed using bioinformatics.The results revealed that the full length of HeNCED gene(GenBank No.PZ350883)was 2 196 bp,with an open reading frame(ORF)of 1 803 bp encoding 600 amino acids.The protein was localized in the cytoplasm,exhibiting acidic,hydrophilic,and unstable properties.Sequence similarity analysis showed over 88%identity with homologous proteins from Gossypium anomalum,G.raimondii,G.arboreum,and H.syriacus,indicating high conservation.qPCR analysis of pod samples at different developmental stages and post-harvest treated with exogenous ABA and nordihydroguaiaretic acid(NDGA)demonstrated a significant positive correlation between HeNCED expression and ABA content(P＜0.01).It was hypothesized that HeNCED regulated secondary wall thickening in H.esculentus pods via modulating ABA synthesis.This study preliminarily revealed the molecular mechanism of HeNCED in Hibiscus esculentus pod aging,which can provide a theoretical basis and genetic resources for molecular breeding,cultivation,and post-harvest storage of H esculentus.