电针干预对近视豚鼠脉络膜血流灌注及PI3K/Akt/mTOR－HIF－1α信号通路的影响OA

•AIM:To study the effects of electroacupuncture(EA)on choroidal blood flow,the phosphatidylinositol 3－ kinase(PI3K)/protein kinase B(Akt)/mammalian target of rapamycin(mTOR)－ hypoxia－ inducible factor 1－ alpha(HIF － 1α)signaling pathway,and ocular biological parameters in form deprivation myopia(FDM)－induced guinea pigs. •METHODS:The guinea pigs were randomly divided into four groups:blank control group;FDM group(subjected to form deprivation to simulate myopia development);FDM+EA group(subjected to form deprivation and treated with EA stimulation);FDM+sham acupuncture group(subjected to form deprivation and given EA stimulation).EA treatments were performed at the Taiyang(EX－HN5)acupoint for the FDM+EA group and at sham acupoints in the FDM+sham group.Measurements included refractive error,axial length(AL),corneal curvature,vitreous diameter,choroidal thickness,and vascular density of the choroid layer.Additionally,the expression levels of PI3K,Akt,mTOR,and HIF－1α mRNA in the sclera were detected. •RESULTS:Eight animals were included in each group.After 4 wk of treatment,the refractive error of the FDM+EA group was significantly reduced compared to the FDM group(P<0.001).The vascular density of the choroid in the FDM+EA group was significantly increased compared to the FDM group(P<0.001).The expression levels of PI3K,Akt,mTOR,and HIF－1α mRNA in the FDM+EA group were significantly reduced compared to the FDM group(P<0.001). •CONCLUSION:EA can improve the vascular density of choroid,antagonize the expression of PI3K/Akt/mTOR－ HIF －1α signaling pathway,effectively improve scleral hypoxia,reduce the diopter of myopic guinea pigs,and play a role in controlling the progression of myopia.