小麦TaDGAT基因克隆及其在花药发育中的潜在功能分析OA

To explore the potential function of diacylglycerol acyltransferase(DGAT)in the develop-ment of anther,the wheat photothermo-sensitive male sterile line BS366 was used as the material.Based on previous transcriptome sequencing results,TaDGAT(TraesCS1A02G125300)was screened and cloned fromBS366.Bioinformatics analysis was employed to predict the structural and functional characteristics of the TaDGAT gene and its encoded protein.The results indicated that the gene has an open reading frame(ORF)of 1 542 bp,encoding 513 amino acids.Based on protein structure,the protein was predicted to be an O-acyltransferase.Multiple sequence alignment and phylogenetic tree analysis revealed that TaDGAT is most closely related to those in wheat and barley.Additionally,miRNAs,such as miR398 and miR169,were found to potentially interact with TaDGAT,participat-ing in lipid metabolism processes and thereby regulating anther development.Protein-protein interac-tion predictions identified key interactions with O-acyltransferase and other critical proteins in lipid metabolism pathways.Subcellular localization prediction indicated that TaDGAT is located in the cell membrane and Golgi apparatus,where it participates in the formation of biological membranes.Ex-pression pattern analysis showed that the expression of TaDGAT in different tissues,the level in an-thers is low,while it is highly expressed under sterile conditions.This also suggested that the gene is closely related to pollen formation.This study provides certain references for elucidating the function of the DGAT gene in wheat.