小麦耐低温相关NAC转录因子分析与表达OA

To investigate the functional characteristics of low-temperature tolerance-related NAC tran-scription factors in wheat,a low-temperature-tolerant variety Mazhamai and a low-temperature-sensi-tive variety Jimai 22 were used for transcriptome sequencing,and 31 candidate NAC transcription fac-tors potentially associated with low-temperature tolerance were screened.Bioinformatics analysis showed that 12 of the TaNAC proteins are basic proteins(pI＞7),while the others are acidic proteins(pI＜7).All proteins are hydrophilic and mainly localized in the nucleus.The secondary structure was dominated by random coils.Collinearity analysis revealed that there were extensive collinear rela-tionships between NAC transcription factors in wheat and rice.Promoter analysis indicated that the promoter regions of these NAC transcription factor genes contained various cis-acting elements involved in stress responses and hormone signaling,among which TaNAC048-A1 harbored the most abundant regulatory elements.Under low-temperature stress,the expression levels of TaNAC048-D1,TaN-AC048-A1,TaNAC015-B4,TaNAC039-D1,TaNAC104-D2,TaNAC048-B1,TaNAC001-B1,and TaN-AC001-A1 were significantly up-regulated in Mazhamai.In Jimai 22,TaNAC048-D1,TaNAC039-D1,and TaNAC104-D2 were significantly up-regulated,while TaNAC048-B1 was significantly down-regu-lated,and the remaining four genes showed no significant changes.The transcriptome sequencing da-ta were verified by qRTPCR.These TaNAC transcription factor genes may play important roles in the response of wheat to low-temperature stress.