小麦-黑麦1BL·1RS易位品种(系)中的1RS结构变异探析OA

To explore the structural differences of 1RS arms in wheat-rye 1BL·1RS translocation culti-vars(lines),the 1BL·1RS translocation cultivar Nanmai 618 was used as parent to hybridize with 131BL·1RS translocation cultivars(lines),including Aikang 58,Chuannong 17 and Lankao Aizao 8,as well as the 7DL·1RS translocation cultivar Lumai 8.A total of 14 types of F1 plants were obtained.High-GC content oligonucleotide probes(Oligo-119.2-2,200G,250G)and high-AT content oligonu-cleotide probes(Oligo-119.2-3,200T,250T)were designed based on the tandem repeats pSc119.2,pSc200,and pSc250.These probes were used in non-denaturing fluorescence in situ hybridization(ND-FISH)analysis for identifying the metaphase chromosomes of root tips from these F1 plants.The results showed that,except for Oligo-119.2-3 which produced no signal,the other five probes generated signals with varying intensities on 1RS chromosome arms from different sources.The sig-nal intensities of probes 200G and 200T on 1RS exhibited consistent variation trends,while the varia-tions reflected by 250G and 250T were inconsistent.For example,250T showed distinct signal inten-sities on 1RS from different sources,whereas 250G displayed similar signal intensities on the corre-sponding 1RS arms.These findings indicated that there were differences in the GC and AT base con-tents of the pSc250 tandem repeated family in 1RS among different cultivars.According to the varia-tion in signal intensity of the probes on 1RS,the 1RS arms in these 15 wheat cultivars(lines)could be classified into four structural types.The results of this study revealed the structural variations of 1RS,which enriches the understanding of 1BL·1RS translocation chromosomes in wheat cultivars(lines)in China.