首荟通便胶囊对功能性便秘大鼠脑肠轴调节作用机制研究OA

Objective This study focuses on exploring the therapeutic effects of Shouhui Tongbian Capsules(首荟通便胶囊)on rats with functional constipation and its potential mechanism of action.Methods Sixty SD rats were randomly allocated into six groups:blank control,model control,positive control,and three Shouhui Tongbian Capsules treatment groups(low-dose:75 mg·kg-1,medium-dose:150 mg·kg-1,high-dose:300 mg·kg-1).Functional constipation models were established in the model and treatment groups via intragastric administration of loperamide hydrochloride.After successful model induction,the treatment groups received their respective doses of Shouhui Tongbian Capsules for 14 days.The fecal moisture content of rat fecal samples were determined,the messenger RNA(mRNA)transcription levels of cAMP-dependent protein kinase(PKA)and cAMP-response element binding protein(CREB)genes in the hippocampus were analyzed by using real-time quantitative reverse transcription polymerase chain reaction(RT-qPCR),Western blot(WB)method was applied to analyze the protein expression levels of PKA and CREB in the hippocampus,immunohistochemical technique was used to evaluate the expression of vasoactive intestinal peptide(VIP)and 5-hydroxytryptamine(5-HT)in colonic tissues.Results Shouhui Tongbian Capsules significantly alleviated symptoms and promoted intestinal motility in rats with loperamide-induced functional constipation.Specifically,it increased fecal water content,ameliorated the pathological state of constipation,enhanced the positive expression intensity of VIP and 5-HT in colon tissue,and up-regulated the mRNA and protein expression levels of PKA and CREB in the hippocampus.Conclusion Shouhui Tongbian Capsules improves intestinal function in constipated rats potentially by modulating the expression of molecules related to the brain-gut axis,the underlying mechanism may be associated with the activation of the PKA/CREB signaling pathway.