六种方法构建大鼠盆腔炎模型的比较研究OA

Objective To compare the pathological morphological changes of rat uterus tissue,the levels of inflammatory factors and adhesion-related molecules in six rat pelvic inflammatory models,and to provide a reference for the selection of animal models of pelvic inflammatory disease and sequelae of pelvic inflammatory disease.Methods 10 rats were randomly selected as the normal group,while the remaining 60 modeling rats were divided into 6 groups,and pelvic inflammatory models were established by six different methods respectively,including foreign body stimulation,phenol glue inflammation,hydrochloric acid inflammation,endogenous pathogens,exogenous and endogenous pathogens,and mechanical injury plus bacterial infection.The pathological changes of uterine tissue were abserved.The levels of IL-1α,IL-6,VEGF,and TGF-β in serum,and the expression of ICAM-1 protein were obtected.Results The IL-1α content in the mechanical injury combined with bacterial infection group,the exogenous and endogenous pathogen co-infection group and the hydrochloric acid-induced inflammation group increased to varying degrees,while the IL-1α content in the other groups did not change significantly;except for the hydrochloric acid-induced inflammation group,the IL-6 content in the other five groups increased to varying degrees;except for the exogenous and endogenous pathogen co-infection group,the VEGF content in the other five groups increased to varying degrees;except for the hydrochloric acid-induced inflammation group and the exogenous and endogenous pathogen co-infection group,the TGF-β content in the other four groups increased to varying degrees;ICAM-1 was strongly positive in all model groups.Pathological results showed,a large amount of connective tissue hyperplasia,endometrial congestion and edema,epithelial cell deformation and necrosis,and inflammatory cell infiltration were observed in the foreign body implantation stimulation group and the phenol mucilage-induced inflammation group;a large amount of abnormal blood flow signals in the muscular layer were observed in the hydrochloric acid-induced inflammation group,and some cells were deformed and necrotic;the endogenous pathogen infection group and the exogenous and endogenous pathogen co-infection group were mainly characterized by increased inflammatory cells.The mechanical injury combined with bacterial infection group showed obvious endometrial congestion and edema,and some epithelial cells were deformed and necrotic.Conclusion All six modeling methods can lead to pelvic inflammatory changes in rats.Among them,the foreign body implantation stimulation group,phenol mucilage-induced inflammation group and hydrochloric acid-induced inflammation group all showed different degrees of sequelae of pelvic inflammatory disease,and the phenol mucilage-induced inflammation group was the most typical;the endogenous pathogen infection group and the exogenous and endogenous pathogen co-infection group all showed different degrees of acute pelvic inflammatory disease,and the exogenous and endogenous pathogen co-infection group was the most typical;the pelvic inflammatory model established by the mechanical injury combined with bacterial infection group was more consistent with the acute attack of sequelae of pelvic inflammatory disease,and was suitable for further exploration of the transformation and prevention between pelvic inflammatory disease and sequelae of pelvic inflammatory disease.