趋化因子CXCL4在斑秃患者外周血中的表达及临床意义OA

Objective The aim of this study was to investigate the expression levels of CXCL4 in the peripheral blood of patients with alopecia areata and to provide evidence for the pathogenesis of alopecia areata and the development of new therapeutic targets.Methods Sixty patients with alopecia areata(alopecia group)and 30 healthy controls(control group)were enrolled.The ex-pression levels of CXCL4 mRNA in the peripheral blood mononuclear cells(PBMCs)were detec-ted by qRT-PCR,and the content of CXCL4 in the serum was detected by ELISA.The relation-ship between CXCL4 expression and age,gender,disease duration,disease activity,and severity(SALT score)was analyzed.Results The expression levels of CXCL4 mRNA in PBMCs of the alopecia group were significantly higher than those of the control group(Z=-3.65,P＜0.001).The expression levels of CXCL4 mRNA in patients with different severity,clinical stages,and dis-ease durations were all higher than those of the control group(all P＜0.05),but there was no significant difference among the subgroups of alopecia areata(all P＞0.05).The content of CX-CL4 in the serum of the alopecia group was significantly higher than that of the control group(Z=-3.90,P＜0.001);the serum CXCL4 levels in each subgroup were also higher than those of the control group(all P＜0.05),with no significant difference among the subgroups of alopecia areata(all P＞0.05).There was no significant correlation between serum CXCL4 protein levels or CXCL4 mRNA levels and the SALT score(P＞0.05).Conclusions The expression levels of CXCL4 in the peripheral blood of alopecia patients are increased,suggesting that it may be in-volved in the pathological process of alopecia.Although it does not significantly correlate with the severity of the disease,it can still be used as a potential auxiliary diagnostic marker and provide a reference for early screening.