基于pc-tNGS技术检测支气管肺泡灌洗液中病原微生物协助病理诊断的价值OA

Objective To evaluate the diagnostic value of probe capture-based targeted next-generation sequenc-ing(pc-tNGS)for detecting pathogenic microorganisms in bronchoalveolar lavage fluid(BALF)from patients with pul-monary infections.Methods BALF samples were collected from 39 patients with pulmonary infections.The micro-bial load and pathogen spectrum were analyzed using pc-tNGS,and the results were compared with those obtained by conventional microbiological testing(CMT)and multiplex PCR-based targeted next-generation sequencing(mp-tNGS).Results The pathogen detection rate of pc-tNGS was significantly higher than that of CMT(97.44%vs 74.36%,P<0.01).In particular,pc-tNGS markedly improved the detection rates of fungi and bacteria compared with CMT(76.92%vs 7.69%,P<0.01).The overall pathogen detection rate of pc-tNGS was comparable to that of mp-tNGS(97.44%vs 97.30%,P>0.05).However,differences in sensitivity for specific pathogens were observed:mp-tNGS showed higher sensitivity for Mycoplasma pneumoniae and Candida species,whereas pc-tNGS demonstrated superior performance in detecting Mycobacterium species.In addition,pc-tNGS simultaneously identified antimicro-bial resistance genes and virulence genes in BALF samples,providing useful information for clinical antimicrobial therapy and subsequent management.Conclusion pc-tNGS is an effective method for pathogen detection in BALF samples from patients with pulmonary infections.Compared with conventional microbiological methods,it provides higher detection efficiency and broader coverage of Mycobacterium species than mp-tNGS.This technique can provide more accurate,comprehensive,and cost-effective etiological diagnostic information,thereby improving the diagnostic efficiency of pulmonary infectious diseases.