烟粉虱传双生病毒的检测与防控技术研究进展OA

The whitefly Bemisia tabaci,a major global agricultural pest,feeds on over 600 plant species and transmits more than 500 plant viruses,posing a continuous threat to global agriculture.Among them,whitefly-transmitted begomoviruses belonging to the genus Begomovirus of the family Geminiviridae are particularly destructive,causing severe yield losses or even complete losses to various important crops such as tomato,cotton and cassava worldwide.In this article,we systematically reviewed the research progress in detection and control technologies for whitefly-transmitted geminiviruses since the beginning of the 21st century.Regarding detection,early efforts predominantly relied on serological techniques such as enzyme-linked immunosorbent assay(ELISA),although their application was constrained by the limited universality of available antibodies.Currently,molecular detection technologies centering on polymerase chain reaction(PCR)have become the mainstream approach.Their derived techniques,including real-time quantitative PCR and multiplex PCR,have significantly enhanced both the accuracy and efficiency of virus detection.Furthermore,isothermal amplification technologies such as rolling circle amplification(RCA),loop-mediated isothermal amplification(LAMP)and recombinase polymerase amplification(RPA)have demonstrated considerable potential for rapid field diagnosis due to their minimal instrumentation requirements.Additionally,next-generation sequencing(NGS)has emerged as a powerful tool for identifying unknown viruses and conducting population genetic studies.In terms of control strategies,we elaborated on an integrated management system guided by the principle of"prevention first,comprehensive control"which spanned the entire crop growth cycle.Prior to planting,emphasis was placed on the selection of resistant varieties and the use of virus-free seedlings,along with the removal of weed hosts and other viral reservoirs.During the cropping period,a combination of agricultural practices(e.g.,crop rotation and intercropping),physical barriers(e.g.,insect-proof nets and sticky traps),biological control(e.g.,natural enemies and microbial agents),and science-based chemical control was employed to suppress whitefly populations and disrupt virus transmission.Following harvest,attention was given to field sanitation to reduce overwintering insect sources and viral vectors.Finally,we presented the following future research directions:(1)In-depth analysis of the complex interaction mechanisms among virus,whitefly and host plants;(2)Development of intelligent and portable detection technologies;and(3)Development of green control technologies such as ecological regulation.This review will provide references and guide for the sustainable management of whitefly-transmitted geminiviruses.