水稻条纹病毒的虫传机制研究进展OA

Rice stripe virus(RSV)is a significant insect-borne plant virus transmitted by the small brown planthopper(Laodelphax striatellus)in a persistent and propagative manner,posing a serious threat to rice production in Asian countries.Within its insect vector,RSV must overcome multiple tissue barriers,e.g.,midgut,salivary glands and ovaries,and meanwhile must evade insect immune defense and regulate tissue microenvironments to achieve both horizontal and vertical transmission,reflecting a complex co-evolutionary relationship between virus and vector.In recent years,the rapid development of molecular biology technologies such as genomic sequencing and small RNA sequencing of L.striatellus,along with molecular interaction studies,has led to significant progress in China in understanding RSV receptor recognition,immune evasion,cross-kingdom regulation and manipulation of vector insect behavior.In this article,we reviewed the key findings in the insect transmission mechanism field of RSV from the past decade,covering:the molecular mechanisms underlying RSV's horizontal and vertical transmission,dynamic interactions between RSV and the vector insect immune system,viral manipulation of vector behavior and metabolism,differential adaptation mechanisms of RSV in insect vector and host plant,and miRNA-mediated cross-kingdom transmission mechanism of virus.Collectively,these studies provide deep insights into the molecular interaction network among RSV,L.striatellus and rice,revealing an asymmetric manipulation-adaptation symbiotic relationship that favors viral transmission.We further proposed that future research should focus on the cellular and molecular mechanisms underlying viral dissemination across insect tissues,the role of symbiotic microbes in modulating transmission efficiency,and the strategies by which RSV manipulates L.striatellus physiology and behavior.Integrating these insights with eco-friendly control approaches such as RNA interference holds promise for developing precise and sustainable strategies to block RSV transmission,and offers new directions for environmentally sound management of arboviral diseases.