细胞外基质靶向性碱性成纤维细胞生长因子/血管生成素-1模拟肽/细胞外基质水凝胶对缺血性脑卒中模型大鼠神经功能恢复的影响OA

Objective To investigate the effects of extracellular matrix(ECM)-targeting basic fibroblast growth factor(EBP-bFGF)/angiopoietin-1 mimetic peptide(AMP)/ECM hydrogel on neurological function recovery in ischemic stroke model rats.Methods Thirty male SD rats aged 6-8 weeks and weighing 250-280 g were used to establish a middle cerebral artery occlusion/reperfusion model.The rats were randomly divided into groups A-C,with 10 rats in each group.Each group received a single 25 μL injection of different hydrogel formulations into the cerebral ischemic region:group A received ECM hydrogel,group B received EBP-bFGF/ECM hydrogel,and group C received EBP-bFGF/AMP/ECM hydrogel.On days 7 and 14 after model estab-lishment,neuromotor function was evaluated using grip strength test,rotarod test,and open field test.On day 14 after model es-tablishment,brain tissues of rats in each group were dissected and isolated,and morphological characteristics were assessed by he-matoxylin-eosin staining and Nissl staining.Immunofluorescence staining was performed to detect the expression levels of neuronal nuclear antigen(NeuN),β-Ⅲ tubulin(Tuj1),von Willebrand factor(vWF),α-smooth muscle actin(α-SMA),vascular endothe-lial cadherin(VE-cadherin),and zonula occludens-1(ZO-1)in brain tissues of rats,to evaluate the status of neurons,blood ves-sels,and the blood-brain barrier.Results On days 7 and 14 after model establishment,compared with groups A and B,rats in group C showed significantly increased grip strength,rotation speed at fall and latency to fall in the rotarod test,as well as signifi-cantly increased center-zone residence time and maximum speed in the open field test(F=7.669-26.840,q=3.911-11.940,P＜0.05).Hematoxylin-eosin and Nissl staining showed that,compared with groups A and B,the brain tissue structure of rats in group C was more intact,with a significantly increased number of Nissl bodies(F=8.520,q=5.604,4.217,P＜0.05).Immunofluorescence staining showed that the numbers of NeuN-and Tuj1-positive neurons,the numbers of vWF-and α-SMA-positive blood vessels,and the mean fluorescence intensities of VE-cadherin and ZO-1 in group C were significantly higher than those in group A;additio-nally,the number of Tuj1-positive neurons,the number of vWF-positive blood vessels,and the mean fluorescence intensity of ZO-1 in group C were significantly higher than those in group B(F=4.350-27.760,q=4.141-10.540,P＜0.05).Conclusion The EBP-bFGF/AMP/ECM hydrogel constructed in this study can effectively promote neuromotor function recovery and neurovas-cular unit regeneration in the infarct area in ischemic stroke model rats.