miR-892b对骨肉瘤细胞增殖、迁移和凋亡的影响及其机制OA

Objective To investigate the effect and mechanism of miR-892b on the proliferation,migration,and apopto-sis of osteosarcoma cells.Methods RT-qPCR was used to measure the relative expression level of miR-892b in osteosarcoma cell lines(MG-63,U2OS)and the human osteoblast cell line hFOB1.19.U2OS cells were divided into group A(transfected with mimic NC),group B(transfected with miR-892b mimic),group C(transfected with inhibitor NC),and group D(transfected with miR-892b inhibitor).CCK-8 assay was used to measure the absorbance value of each group at 24,48,and 72 hours of culture after transfection;Transwell assay was used to measure the number of migrating cells in each group;Western blot was used to measure the expression levels of the apoptosis-related protein Bax and the key protein p-NF-κB in the NF-κB signaling pathway.Results RT-qPCR showed that compared with the hFOB1.19 cells,the MG-63 and U2OS cells showed a significant reduction in the relative expression level of miR-892b(F=73.59,tDunnett=7.83,9.92,P＜0.05).CCK-8 assay showed that at 24,48,and 72 hours of cul-ture,group B had a significantly lower absorbance value than group A,while group D had a significantly higher absorbance value than group C(F=5.91-31.23,P＜0.05).Compared with group A,group B had a significant reduction in the number of migrating cells,a significant increase in the protein expression level of Bax,and a significant reduction in the protein expression level of p-NF-κB;compared with group C,group D had a significant increase in the number of migrating cells,a significant reduction in the pro-tein expression level of Bax,and a significant increase in the protein expression level of p-NF-κB(t=3.64-7.16,P＜0.05).Con-clusion There is a relatively low expression level of miR-892b in osteosarcoma cells,and miR-892b may regulate the proliferation,migration,and apoptosis of osteosarcoma cells by affecting the NF-κB pathway.