核不均一核糖核蛋白U对非小细胞肺癌细胞迁移和增殖的影响OA

Objective To investigate the effect of heterogeneous nuclear ribonucleoprotein U(hnRNP U)on the migra-tion and proliferation of non-small cell lung cancer(NSCLC)cells.Methods Based on the TCGA database,a pan-cancer analy-sis was performed for the expression level of the hnRNP U gene in various tumor tissues.The datasets of patients with NSCLC subtypes of lung adenocarcinoma(LUAD)and lung squamous cell carcinoma(LUSC)were collected,and the Kaplan-Meier curves was used for survival analysis.Western blot was used to measure the relative protein expression level of hnRNP U in NSCLC cell lines(H460,A549,H1299,Anip973,PC9)and the human normal lung epithelial cell line Beas-2B.H1299 cells were divided into groups A and B,and A549 cells were divided into groups C and D.The cells in groups A and C were transfected with an empty Myc vector,while those in groups B and D were transfected with a Myc-hnRNP U overexpression plasmid.Cell scratch assay and plate colony formation assay were used to measure the migration and proliferation abilities of cells in each group.Results The analysis based on the TCGA database showed that in the patients with LUAD and LUSC,the expression level of hnRNP U in tumor tissue was significantly higher than that in the corresponding adjacent normal tissue(P＜0.05),and the expression level of hnRNP U in tumor tissue was significantly negatively correlated with the disease-free survival rate and overall survival rate of LUAD patients and the overall survival rate of LUSC patients(P＜0.05).The results of Western blot showed that compared with Beas-2B cells,there was a significant increase in the relative protein expression level of hnRNP U in A549,Anip973,H460,and H1299 cells(F=28.118,tLSD=-8.576--2.462,P＜0.05).The results of cell scratch assay and plate colony formation assay showed that compared with group A,group B had significant increases in cell migration rate and the number of plate colonies,and compared with group C,group D had significant increases in these two indicators(t=-13.539-3.859,P＜0.05).Conclusion The ex-pression of hnRNP U is upregulated in both the tumor tissue of NSCLC patients and NSCLC cell lines,and the high expression of hnRNP U can promote the migration and proliferation of NSCLC cells.