铁死亡相关基因作为克罗恩病诊断生物标志物的价值OA

Objective To explore the value of ferroptosis-related genes as diagnostic biomarkers for Crohn's disease(CD).Methods Three gene expression matrices related to CD were selected from the GEO data-base.After excluding specimens from non-ileal parts of the sequenced tissues,a total of 402 CD patients were included as the CD group and 63 healthy individuals as the control group.Additionally,12 CD patients who visited the hospital from January 2022 to December 2024 were selected as the clinical CD group,among which 8 patients also had normal intestinal mucosal tissues without inflammation adjacent to the lesion,which were frozen and preserved for analysis as the paired control group.The three CD-related transcriptome datasets were downloaded from the GEO database and integrated after batch effect correction.The differentially ex-pressed genes(DEGs)between CD and healthy controls were screened,and the intersection with ferroptosis-related genes was taken to obtain the key common genes.Functional enrichment analysis,protein interaction network construction and core gene expression verification were carried out,and six algorithms were utilized to sort the genes.The diagnostic value of six genes that ranked highly and consistently across all six algo-rithms for CD was analyzed using the receiver operating characteristic(ROC)curve.Results A total of 607 DEGs were screened out,and 32 key common genes were identified,among which 16 were driver genes,14 were inhibitory genes,and 2 were marker genes.The Kyoto encyclopedia of genes and genomes enrichment a-nalysis showed that the top 10 significant pathways mainly involved ferroptosis,fatty acid synthesis,ubiqui-none and terpenoid synthesis,African trypanosomiasis,antifolate resistance,fatty acid degradation,graft-ver-sus-host disease,arginine synthesis,interleukin-17(IL-17)signaling pathway and malaria.Six key hub genes including glutathione peroxidase 4(GPX4),hypoxia-inducible factor-1α(HIF-1α),interleukin-1β(IL-1β),in-terleukin-6(IL-6),lipoprotein transporter 2(LCN2)and prostaglandin endoperoxide synthase 2(PTGS2)were screened out through the protein-protein interaction network.The messenger RNA(mRNA)level of GPX4 in the CD group was lower than that in the control group,while the mRNA levels of HIF-1α,IL-1β,IL-6,LCN2 and PTGS2 were higher than those in the control group,and the differences were statistically signifi-cant(P＜0.05).The ROC curve analysis showed that the areas under the curve(AUC)for GPX4,HIF-1α,IL-1β,IL-6,LCN2 and PTGS2 alone for diagnosing CD were 0.776,0.826,0.853,0.716,0.891 and 0.780 re-spectively.The GPX4 mRNA level in the clinical CD group was lower than that in the paired control group,while the levels of HIF-1α mRNA,IL-1β mRNA,IL-6 mRNA,LCN2 mRNA and PTGS2 mRNA were higher than those in the paired control group,and the differences were statistically significant(P＜0.05).Conclusion This study systematically identifies the key genes involved in ferroptosis that are differentially expressed in CD,reveals their potential disease mechanisms,and has good diagnostic efficacy.It can provide new ideas and candidate targets for the precise diagnosis and treatment of CD.