产自四川的环纹硫黄菌的生物学特性OA

A pure culture strain(SSJ01)was obtained by isolating and purifying an orange edible mushroom purchased from a market in Kangding City,Garzê Tibetan Autonomous Prefecture,Sichuan Province,and was identified as Laetiporus zonatus based on morphological characteristics and ITS sequence analysis.The mycelial growth conditions of this fungus were optimized through single-factor and orthogonal experiments,and a preliminary domestication study was conducted.The results of the single-factor experiments indicated that the optimal carbon source for mycelial growth was sucrose,the optimal nitrogen source was yeast extract,the optimal C/N was 30:1,the optimal temperature was 25℃,the optimal pH was 5.0,and the optimal light conditions were complete darkness;no inorganic salts were required.The orthogonal experiment showed that the effects of the tested factors on mycelial growth rate were ranked as temperature>pH>carbon source>nitrogen source.The optimal combination of culture condition was selecting fructose as the carbon source and peptone as the nitrogen source,and cultivation under 25℃and pH 5.0.For domestication cultivation,substrate composed of 94%cottonseed hulls,5%wheat bran,and 1%lime with moisture content of 55%-60%was adopted.After 30-35 day incubation in the dark at 25℃,the plastic cultivation bags fully colonised by mycelium were transferred to the conditions of 20-25℃,85%-90%relative humidity,illumination of about 12 hours under diffused light and ventilation of three times per day.In about 20-30 days clustered early fruiting bodies formed.This research provides basic data for further studies of domestication,cultivation,development and utilization of Laetiporus zonatus.