二陈汤治疗分泌性中耳炎潜在机制的网络药理学与分子对接研究OA

Objective To systematically explore the potential active ingredients,key therapeutic targets,and molecular mechanism of Erchen Decoction in the treatment of secretory otitis media(SOM)based on network pharmacology and molecular docking techniques.Methods The main ac-tive ingredients and predicted targets of Erchen Decoction were screened through the TCMSP database.SOM-related targets were obtained from the DisGeNET,GeneCards,and OMIM databases.The inter-secting targets were used to construct a protein-protein interaction(PPI)network and screen for core targets.Gene ontology(GO)functional annotation and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analysis were employed to elucidate their biological functions.A"tradi-tional Chinese medicine-active ingredient-target"network was constructed to screen for major active in-gredients,and molecular docking was performed to validate the interactions between core targets and key ingredients.Results A total of 87 common targets between Erchen Decoction and SOM were identified.PPI network analysis revealed that interleukin-6(IL-6),tumor necrosis factor(TNF),protein kinase B(AKT1),and interleukin-1 β(IL-1β)were core targets.GO functional annotation and KEGG enrichment analysis suggested that the targets were primarily involved in inflammatory responses,oxidative stress regulation,and signaling pathways such as advanced glycation end products and their receptors(AGE-RAGE).Network analysis indicated that quercetin,naringenin,kaempferol,7-me-thoxy-2-methylisoflavone,and formononetin were key active ingredients.Molecular docking demon-strated strong binding capabilities between these ingredients and the core targets.Conclusion The main active substances in Erchen Decoction,namely quercetin,naringenin,kaempferol,7-methoxy-2-methylisoflavone,and formononetin,primarily treat SOM by mediating key targets such as IL-6,TNF,AKT1,and IL-1 β to regulate inflammatory responses,oxidative stress,and the AGE-RAGE pathway.