石胡荽来源的miR396a-5p对鼻咽癌细胞增殖及迁移的作用及其机制OA

Objective To detect the expression profile of Centipeda minima miRNA,explore the expression levels and secondary structures of high-abundance miRNAs,and investigate their role in the proliferation and migration of nasopharyngeal carcinoma cells.Methods The miRNA expression profile of Centipeda minima was constructed by miRNA high-throughput sequencing.Bioinformatics tools were used to compare the sequence and structural differences between miRNA from Centipeda minima and those from closely related species.The downstream target genes of miRNA were identified using target gene prediction,transcriptome sequencing analysis and dual-luciferase reporter gene assays.The C666-1 human nasopharyngeal carcinoma cell line was used to analyze the effects of Centipeda minima miRNA on cell proliferation and migration by means of cell viability and scratch assays.Results The miRNA expression profile of Centipeda minima was analyzed by high-throughput sequencing technology.A total of 243 miRNAs were identified,among which 13 were novel miRNA unique to this plant,and the secondary structures of miRNA precursors were predicted.Since miR396a-5p,which is expressed in the highest abundance in Centipeda minima,has unique mature and precursor sequences and structures,we named it cmi-miR396a-5p.cmi-miR396a-5p was predicted to target abundant homologous target genes in cancer-related pathways,indicating significant anti-tumor regulatory potential.The dual-luciferase reporter gene results showed that cmi-miR396a-5p directly acted on the 3'untranslated region(3'UTR)of STAT3,a gene related to the progression of nasopharyngeal carcinoma,and inhibited the mRNA and protein expression of STAT3.cmi-miR396a-5p could down-regulate Cyclin D1 and B-cell lymphoma/leukemia-2 protein(Bcl-2),downstream targets of STAT3,promote cell apoptosis,and significantly inhibit the in vitro proliferation and migration ability of C666-1 nasopharyngeal carcinoma cells.Conclusion This study revealed the miRNA expression profile of Centipeda minima.Among them,cmi-miR396a-5p derived from Centipeda minima can exert significant anti-nasopharyngeal carcinoma activity by targeting and negatively regulating STAT3.