龟板固本方对环磷酰胺诱导小鼠免疫抑制的改善作用OA

Objective To explore the immunomodulatory effect of Guiban Guben Formula(GBGBF)on cyclophosphamide(CTX)-induced immunosuppressed mice.Methods C57BL/6J mice were randomly divided by body weight into normal control group,model group,thymosin group(0.2 mL/d),low-dose GBGBF group(0.5 mL/d),and high-dose GBGBF group(1.0 mL/d),with 10 mice per group.The thymosin group received intraperitoneal injections of the corresponding dose of thymosin;the low-and high-dose GBGBF groups were administered the corresponding doses of the GBGBF via gavage;and the normal control and model groups were administered the same volume of saline via gavage,once daily for 7 consecutive days.Except for the normal control group,mice in the other groups were intraperitoneally injected with CTX at a dose of 80 mg/kg·d-1 for the last 3 days to establish an immunosuppressed mouse model.Body weight,spleen index,thymus index,and liver index were measured for each group.An automatic hematology analyzer was used to determine the counts of white blood cells,red blood cells,and platelets.Wright's staining was performed to measure macrophage phagocytic rate and phagocytic index.The MTT assay was used to evaluate T lymphocyte stimulation index.Lactate dehydrogenase(LDH)assay was conducted to check natural killer(NK)cell cytotoxic activity.ELISA was employed to determine serum levels of immunoglobulin G(IgG),immunoglobulin M(IgM),interleukin(IL)-3,IL-1β,IL-4,IL-6,and interferon-γ(IFN-γ)in each group.Results Compared with the normal control group,the model group exhibited significant decreases in body weight,spleen index,thymus index,liver index,white blood cell count,red blood cell count,platelet count,macrophage phagocytic rate,macrophage phagocytic index,T lymphocyte stimulation index,and NK cell cytotoxic activity,as well as serum levels of IgG,IgM,IL-3,IL-1β,IL-4,IL-6,and IFN-γ(P＜0.01).Compared with the model group,both the thymosin group and the high-dose GBGBF groups showed significant increases in body weight,spleen index,thymus index,and liver index,white blood cell count,red blood cell count,platelet count,macrophage phagocytic rate,macrophage phagocytic index,T lymphocyte stimulation index,and NK cell cytotoxic activity,as well as serum levels of IgG,IgM,IL-3,IL-1β,IL-4,IL-6,and IFN-γ(P＜0.05,P＜0.01).Compared with the thymosin group,the low-dose GBGBF group showed significantly lower body weight,white blood cell count,platelet count,macrophage phagocytic rate,macrophage phagocytic index,T lymphocyte stimulation index,NK cell cytotoxic activity,and serum IgM levels(P＜0.05,P＜0.01).Compared with the low-dose GBGBF group,the high-dose GBGBF group demonstrated significant increases in body weight,spleen index,thymus index,liver index,white blood cell count,platelet count,macrophage phagocytic rate,macrophage phagocytic index,T lymphocyte stimulation index,NK cell cytotoxic activity,and serum levels IgG and IgM(P＜0.05,P＜0.01).Conclusion GBGBF effectively enhances the immune function of CTX-induced immun-osuppressed mice by improving immune system-related indicators.