基于稳定同位素技术的不同产地蚕茧地理溯源OA

Geographic traceability of silkworm cocoons is a critical component of the traceability system for characteristic agricultural products,playing a significant role in protecting regional brands.To investigate the feasibility of applying stable isotope technology to the geographic traceability of silkworm cocoons,this study determined the hydrogen,oxygen,carbon,and nitrogen stable isotope ratios in silkworm cocoons from multiple provinces and constructed geographic traceability models using different chemometric methods.The analysis of isotope ratios revealed varying degrees of regional differences in δ2H,δ18O,δ13C,and 815N among silkworm cocoons from different provinces.Hierarchical cluster analysis indicated that silkworm cocoon samples could be clustered according to their geographical origins.Principal component analysis demonstrated a spatial separation characteristic among samples from Sichuan and those from Zhejiang,Jiangsu,and Shandong.In the linear discriminant analysis model,the original discriminant accuracy and cross-validation discriminant accuracy were 77.4％and 72.9％respectively,with the highest accuracy achieved for samples from Sichuan,which were distinctly differentiated from those collected from coastal provinces.The results showed that stable isotope technology combined with chemometric methods can effectively discriminate the geographical origin of silkworm cocoons,providing a scientific basis for improving the traceability technology system of agricultural products.