结球甘蓝中心柱长度性状全基因组关联分析及候选基因预测OA

Core length is a key factor influencing the market value of head cabbage(B.oleracea var.capitata L.).To elucidate the genetic mechanisms and identify the regulatory genes,this study measured the ratio of core length to head height across 52 accessions,which exhibited a normal distribution.Based on this ratio and resequencing-derived single nucleotide polymorphism(SNP)makers,a genome-wide association study(GWAS)was conducted.Totally eight SNPs associated with core length were identified,distributed across chromosomes C03,C06,C07,and C09.Notably,three of these associated SNPs were clustered within an interval of 65 594 673-65 622 331 bp on chromosome C03.Candidate gene annotation of all associated regions yielded 30 candidate genes.Among them,BolC03g077160.2J,BolC09g005220.2J,BolC09g005230.2J,and BolC09g005240.2J are involved in gibberellin biosynthesis and flowering regulation.BolC03g077160.2J is homolog of Arabidopsis thaliana gene At4g21690,which encodes gibberellin 3-beta-dioxygenase 3-an enzyme that catalyzes the conversion of inactive gibberellins precursors to biologically active forms.BolC03g077160.2J was highly expressed in flowers and buds.Exogenous gibberellin treatment significantly promoted core elongation in head cabbage,providing direct physiological evidence supporting the involvement of endogenous gibberellin metabolism-related genes in modulating core length.Therefore,this study provides candidate genetic resources for molecular breeding aimed at optimizing core length.