大别山牛背最长肌脂肪酸差异相关的基因可变剪接分析OA

In order to identify and analyze the alternative splicing(AS)events involved in the differential regulation of fatty acids in the longest dorsal muscle of Dabieshan cattle,high-throughput transcriptome sequencing was used to analyze AS events in longissimus dorsi samples with high fatty acid(HF)and low fatty acid(LF),and differentially spliced genes(DSGs)were screened.GO functional annotation and KEGG signaling pathway enrichment analysis were performed on DSGs,and DSGs involved in key KEGG signaling pathways were screened for protein-protein interaction(PPI)network analysis.The results showed that a total of 26,499 AS events were identified,of which 77.43%were exon skipping events.A total of 682 differential AS events were screened,involving 546 differential splicing genes(DSGs).The DSGs mainly participated in metabolic processes,muscle development,and fatty acid metabolism,and were enriched in key signaling pathways such as AMPK and PPAR.The key genes UBC,SMAD2,RHOA,PI4K2B,and TRAF6 played important roles in the regulation of fatty acid metabolism.The results indicated that differential alternative splicing might be involved in regulating the deposition of fatty acids in the longest dorsal muscle of Dabieshan cattle through the interaction of multiple genes.