不同耐盐作物对根际土壤化学性质及古菌群落的影响OA

In order to promote the screening of salt-tolerant crops in saline-alkali land and the utilization of soil microbial resources,this study selected four salt-tolerant crops:cotton,sea blite,alfalfa,and soybean,along with an unplanted control.A pot experiment was conducted to analyze the chemical properties and archaeal community characteristics of the rhizosphere soil of these four crops,to further elucidate the effects of different salt-tolerant crops on rhizosphere soil chemical properties and archaeal communities.The results showed that the pH and EC of the rhizosphere soil were significantly lower(P≤0.05)in all four salt-tolerant crops compared to the control.Alfalfa and soybean significantly increased the TN content of the rhizosphere soil,and alfalfa also increased the TC content.High-throughput sequencing analysis revealed that the dominant archaeal phyla common to the rhizosphere soil of different salt-tolerant crops were Thaumarchaeota and Euryarchaeota.The dominant archaeal genera were Nitrososphaera,Methanosarcina,Methanoculleus,Natronococcus,Haloterrigena,Haloferax,and Halococcus.The Shannon index of the archaeal community in the sea blite treatment was significantly higher than that in other treatments.The Chao1 index in the alfalfa treatment was significantly higher than that in the control and sea blite treatments,but showed no significant difference compared to the cotton and soybean treatments.Soil TN content,C/N ratio,SWC,and Na+content were identified as key soil factors regulating the composition of the archaeal community structure.In conclusion,sea blite significantly improved the saline environment of the rhizosphere soil and enhanced archaeal community diversity,while soybean and alfalfa increased the rhizosphere soil TN content,with alfalfa also boosting soil TC content and improving archaeal species richness.