不同鳞伞菌株的分子鉴定与栽培特性研究OA

In order to develop wild Pholiota species resources and clarify strain characteristics,providing theoretical basis for future variety breeding and artificial large-scale production,research was conducted on 10 similar Pholiota strains collected from different regions of Heilongjiang Province.The study investigated mycelial growth characteristics,mycelial microstructure,spore morphological features,rDNA ITS sequence analysis and identification,as well as cultivation traits.Results demonstrated that all tested strains exhibited circular colonies with concentric wheel patterns,dense mycelium with uniform margins,and typical lock-like junctions.The strains could be classified into robust and slender types,showing significant differences in colony color and growth rate.Through ITS sequence alignment and phylogenetic analysis,six strains(HS-1,HS-2,HS-3,HS-8,HS-9,HS-10)were identified as Pholiota adiposa,while four strains(HS-4,HS-5,HS-6,HS-7)were identified as Pholiota limonella.Under conditions of 20℃ and 90％humidity,four strains(HS-1,HS-3,HS-7,HS-9)successfully produced fruiting bodies with significant morphological variations.Among them,HS-1 and HS-9 exhibited superior comprehensive agronomic traits,and all four fruiting strains demonstrated high spore maturity and good viability.