Box-Behnken法优化锦灯笼总黄酮提取工艺及多产地质量评价OA

In order to promote the development and utilization of the resources of Physalis alkekengi L.and its quality control,using P.alkekengi L.as the raw material,total flavonoids are extracted from it by ultrasonic-assisted extraction technology.Single-factor experiments were conducted to investigate the effects of three variables,i.e.,extraction time(A),ethanol volume fraction(B),and solid-liquid ratio(C),on the yield of total flavonoids.Subsequently,Box-Behnken response surface methodology(RSM)was employed to optimize the extraction process of TFs from P.alkekengi L.Samples of P.alkekengi L.collected from ten producing areas were subjected to macroscopic identification,microscopic identification,thin-layer chromatography(TLC)identification,as well as determinations of moisture content,ethanol-soluble extract content,and TF content,so as to comparatively evaluate the quality of P.alkekengi L.from different producing areas.The results demonstrated that response surface analysis revealed the order of influence of each factor on the TF yield from the whole fruit of P.alkekengi L.was B＞A＞C.The optimal extraction process parameters were an extraction time of 40.823 min,an ethanol concentration of 79.378％,and a solid-liquid ratio of l:30.704.Under these parameters,the yield of total flavonoids(TFs)from P.alkekengi L.was 0.567％.Microscopic identification showed that the parenchyma tissue of P.alkekengi L.powder from Jilin Province contained abundant large orange-red particles with uniform distribution.TLC identification indicated that the spots of P.alkekengi L.samples from 10 different producing areas,the reference crude drug of P.alkekengi L.,and physalin L were clear with good resolution.All determined moisture contents complied with the specifications of the Chinese Pharmacopoeia(ChP).The order of ethanol-soluble extract content from high to low was,Jilin＞Inner Mongolia＞Sichuan＞Liaoning＞Zhejiang＞Hebei＞Heilongjiang＞Henan＞Hubei＞Shandong.TF content determination results showed that the contents in samples from Jilin and Sichuan were similar and the highest among all tested samples.In conclusion,the Box-Behnken method was used to construct a multi-factor interaction model to optimize the extraction process of P.alkekengi and compare the quality of P.alkekengi from different origins.