阴道黏液电阻值动态变化在梅花鹿同期发情监测及最佳输精时机判定中的应用OA
Application of dynamic changes in vaginal mucus resistance for estrus monitoring and optimal timing of sperm insemination in sika deer
为探索梅花鹿母鹿同期发情时阴道黏液电阻值在发情检测与最佳输精时机,试验以56只 健康梅花母鹿为对象,采用阴道外源孕酮缓释栓(CIDR)+孕马血清促性腺激素(PMSG)+促黄体生成素释放激素A3(LHRH-A3)同期发情方案,于埋栓(0 d)、撤栓(12 d)、人工输精(15 d)及输精后监测(24 d)4个时间点,用阴道电解质测定仪记录阴道黏液电阻值数据,并以血清生殖激素水平为母鹿发情状态参照,探求发情周期母鹿的阴道黏液电阻值变化规律.结果表明:在发情高峰期(15 d)电阻值最低,为(325.00±48.69)Ω,极显著低于其他3个阶段(P<0.01);母鹿阴道黏液电阻值低谷与(E2)、(LH)水平峰值及(P4)水平低谷同步出现,呈现典型的发情期激素模式.说明监测阴道黏液电阻值变化能够及时地判定梅花鹿的发情状态及最佳输精时机.
To investigate the practical effects of vaginal mucus electrical resistance(VER)used for estrus detection and optimal timing of artificial insemination in female sika deer,fifty-six healthy does were synchronized using a CIDR+PMSG+LHRH-A3 protocol.VER values were measured with a vaginal electrolyte analyzer at four time points:CIDR insertion(day 0),CIDR withdrawal(day 12),artificial insemination(day 15)and post-insemination monitoring(day 24).Serum levels of estradiol(E₂),luteinizing hormone(LH)and progesterone(P₄)were used as hormonal references.At the estrus peak(day 15),VER reached its nadir[(325±48.69)Ω],which was significantly lower than at the other three stages(P<0.01).The VER nadir coincided with the peaks of E₂ and LH and the nadir of P₄,showing a typical estrous hormone profile.These findings demonstrate that monitoring VER changes could provide a reliable and timely method for assessing estrus status and determining the optimal insemination time in sika deer.
张旺;马瑜锴;商业;姜淇;郭行健;李和平
东北林业大学野生动物与自然保护地学院,黑龙江 哈尔滨 150040东北林业大学野生动物与自然保护地学院,黑龙江 哈尔滨 150040辽阳千山呈龙科技公司,辽宁 辽阳 111220东北林业大学野生动物与自然保护地学院,黑龙江 哈尔滨 150040东北林业大学野生动物与自然保护地学院,黑龙江 哈尔滨 150040东北林业大学野生动物与自然保护地学院,黑龙江 哈尔滨 150040
农业科技
梅花鹿同期发情阴道黏液电阻值输精时机生殖激素
sika deerestrus synchronizationvaginal mucus electrical resistance(VER)insemination timingreproductive hormones
《黑龙江动物繁殖》 2026 (2)
45-49,5
国家重点研发计划项目(2023YFD1302001)
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