牛羊卵母细胞体外成熟技术研究的文献分析OA

To understand the current research progress in in vitro maturation(IVM)technology of bovine and ovine oocytes,this study conducted keyword co-occurrence,density,and trending topic analyses using vosviewer and bibliometrix based on relevant literature published from 2015 to 2025.The results showed that current research on bovine and ovine oocyte IVM commonly focuses on key issues including insufficient follicular microenvironment support,enhanced oxidative stress,mitochondrial dysfunction,increased apoptosis,and vitrification-induced damage.Specifically,bovine studies emphasize microenvironment reconstruction,extracellular vesicle-mediated communication,and molecular quality evaluation,whereas ovine studies concentrate more on cryopreservation vulnerability,antioxidant protection,and IVM quality improvement of oocytes from different sources.In conclusion,the core bottleneck of bovine and ovine oocyte IVM lies in the difficulty of fully reconstructing the in vivo follicular microenvironment in vitro;future optimization should develop more species-specific culture strategies and multidimensional quality evaluation systems based on a comprehensive understanding of shared mechanisms.