利木赞牛超数排卵及胚胎移植影响因素探究OA

To investigate the factors influencing the effects of superovulation and embryo transfer in Limousin cattle,a total of 8 Limousin donor cows and 26 Simmental recipient cows were used in this study.Donor cows were superovulated with a CIDR+FSH+PG+GnRH protocol,and recipient cows were synchronized using a GnRH+PG regimen.The numbers of follicles and corpora lutea were determined by B-ultrasonography and rectal palpation.Embryo collection and transfer were conducted by non-surgical methods.Data were statistically analyzed using Spearman rank correlation and Fisher's exact test.The results showed that the number of corpora lutea on the day of embryo flushing was significantly and strongly positively correlated with the number of recovered embryos(rS=0.868,P=0.006).The number of pre-superovulation ovarian follicles in donor cows showed a positive but non-significant tendency with embryo yield(rS=0.476,P=0.236).After estrus synchronization,the pregnancy rate of recipient cows with grade A corpora lutea was 61.11%(11/18),and that with grade B corpora lutea was 50.00%(4/8),showing no significant difference(P=0.683).The pregnancy rate for morula transfer was 52.63%(10/19),and 71.43%(5/7)for blastocyst transfer,showing no significant difference(P=0.658).In conclusion,the number of corpora lutea in donor cows can be an indicator of the embryo production potential.Corpus luteum quality and embryo type in recipient cows have no significant effects on pregnancy rate.