胚胎移植当日受体母羊血清代谢组特征与妊娠结局的关联研究OA

This study aimed to preliminarily explore the association between serum metabolic characteristics of recipient ewes on the day of embryo transfer and subsequent pregnancy outcome.Healthy Mongolian sheep were used as the experimental animals.On the day of embryo transfer,jugular venous blood was collected,and recipient ewes were classified into pregnant group(n=7)and non-pregnant group(n=3)according to pregnancy diagnosis results.Non-targeted metabolomics analysis of serum samples was performed using liquid chromatography tandem mass spectrometry(LC-MS/MS)technology,and principal component analysis(PCA)and orthogonal partial least squares discriminant analysis(OPLS-DA)models were used for data analysis.The results showed that a total of 21 differential metabolites(VIP>1,P<0.05,FC>2or<0.5)were identified,mainly including S-allyl-L-cysteine,L-alanyl-L-alanyl-L-arginine,glycine dehydrocholic acid,and arachidic acid.KEGG pathway enrichment analysis showed that differential metabolites mainly involved pathways such as biosynthesis of unsaturated fatty acids,vitamin B₆ metabolism,purine metabolism,and biosynthesis of cofactors.There is a certain correlation between the serum metabolomic characteristics of recipient ewes on the day of embryo transfer and pregnancy outcomes.Metabolites such as S-allyl-L-cysteine,L-alanyl-L-alanyl-L-arginine,glycine dehydrocholic acid,and arachidic acid suggest that they are candidate biomarkers for evaluating the pregnancy potential of recipient ewes.