利用功能型添加剂实现甲醇废水厌氧处理过程中污泥的快速颗粒化OA

Although methanol is highly biodegradable,its efficient and stable anaerobic treatment remains constrained by several factors,including the prolonged adaptation period of microorganisms to toxic substances,a monolithic microbial community structure,and insufficient sludge granulation.In this study,two types of functional supplements were applied to achieve the rapid start-up of two upflow anaerobic sludge blanket(UASB)reactors:1)Sodium bicarbonate as an inorganic carbon source(IC),and 2)An amino acid-rich organic functional supplement(FS).UASB-A was supplemented with 3 000  mg·L-1 sodium bicarbonate and 127 mg·L-1 FS,while UASB-B received 3 000 mg·L-1 sodium bicarbonate.The results demonstrated that both types of functional supplements effectively promoted the rapid start-up of the UASB reactors and the formation of anaerobic granular sludge by shortening the hydraulic retention time(HRT)and increasing the organic loading rate(4,6,9,15,20,and 30 g COD·L-1·d-1).These effects were reflected in significant improvements in the total suspended solids(TSS),volatile suspended solids(VSS)concentrations,and sludge particle size distribution.Microbial community analysis revealed that under an HRT of 0.2 d,the relative abundance of Acetobacterium reached its peak,at 30.2％in UASB-A and 36.9％in UASB-B.The addition of sodium bicarbonate promoted the synergistic interaction between the acetogen Acetobacterium and the aceticlastic methanogen Methanothrix,while the addition of FS significantly increased the abundance of the acetogen Sporomusa,which could establish new mutualistic interactions with Methanothrix,thereby facilitating the sludge granulation process.