利用回交重组自交系定位水稻剑叶和穗部性状主效QTLsOA

In order to explore the genetic regulation mechanism of flag leaf and panicle traits in rice(Oryza sativa L.)and identify the main quantitative trait loci(QTLs),a backcross recombinant inbred lines(BILs)derived from the hybridization-backcross of Habata-ki and Sasanishiki was used as the experimental material to identify the main QTLs of flag leaf and panicle traits in two environments.The results showed that there were significant differences in flag leaf and panicle traits between parents under different environments.The related traits of BILs varied greatly,showing an approximate normal continuous distribution.There was a significant correlation be-tween panicle traits,and flag leaf traits had an important influence on panicle traits.A total of 42 QTLs were detected,with 7 and 35 QTLs identified for flag leaf traits and panicle traits,respectively.The related loci were distributed on chromosomes 1-10 and 12.Indi-vidual QTL explained 7.12%-33.96%of the phenotypic variation,and the additive effect was-20.86-20.88.Among them,qPL5,qGN1.1,qGN5,qSN5.1,qSSR6,qSSR12 and qTGW9 were stably expressed in different environments.The QTLs related to flag leaf traits and panicle traits were clustered on chromosomes.42 QTLs were distributed on 19 chromosome segments,forming 5 major QTL clusters,including qFLP1,qFLP2,qFLP5,qFLP9 and qFLP12.Among them,the stably expressed major QTL cluster qFLP5 had ob-vious regulatory effects on flag leaf width,panicle length,panicle weight,spikelet number and filled grain number,which was worthy of further study.